Primer pool recipe
Prepare two primer pools by mixing equal volumes of each primer. To help normalize sequencing coverage, different primers should be pooled at different concentrations. Below you will find which primers should be pooled at 10 uM, 50uM, or 100uM.
The following primers should be pooled at a concentration of 10 uM:
| Pool 1 | |
|---|---|
| 400_1_out_L | 400_1_out_R |
| 400_5_out_L | 400_5_out_R |
| 400_7_out_L | 400_7_out_R |
| 400_11_out_L | 400_11_out_R |
| 400_13_out_L | 400_13_out_R |
| 400_15_out_L | 400_15_out_R |
| 400_19_out_L | 400_19_out_R |
| 400_23_out_L | 400_23_out_R |
| 400_27_out_L | 400_27_out_R |
| 400_29_out_L | 400_29_out_R |
| 400_31_out_L | 400_31_out_R |
| 400_35_out_L | 400_35_out_R |
| Pool 2 | |
|---|---|
| 400_2_out_L | 400_2_out_R |
| 400_4_out_L | 400_4_out_R |
| 400_6_out_L | 400_6_out_R |
| 400_10_out_L | 400_10_out_R |
| 400_12_out_L | 400_12_out_R |
| 400_26_out_L | 400_26_out_R |
| 400_28_out_L | 400_28_out_R |
| 400_30_out_L | 400_30_out_R |
| 400_32_out_L | 400_32_out_R |
These primers should be pooled at a concentration of 50uM:
| Pool 1 | |
|---|---|
| 400_3_out_L | 400_3_out_R |
| 400_9_out_L | 400_9_out_R |
| 400_21_out_L | 400_21_out_R |
| 400_33_out_L | 400_33_out_R |
| Pool 2 | |
|---|---|
| 400_8_out_L | 400_8_out_R |
| 400_14_out_L | 400_14_out_R |
| 400_20_out_L | 400_20_out_R |
| 400_16_out_L | 400_16_out_R |
| 400_34_out_L | 400_34_out_R |
These primers should be pooled at a concentration of 100uM:
| Pool 1 | |
|---|---|
| 400_17_out_L | 400_17_out_R |
| 400_25_out_L | 400_25_out_R |
| Pool 2 | |
|---|---|
| 400_18_out_L | 400_18_out_R |
| 400_22_out_L | 400_22_out_R |
| 400_24_out_L | 400_24_out_R |