1. fauna
  2. builds
  3. DENGUE

RethinkDB database to support real-time virus analysis
Source code
github.com/nextstrain/fauna
Contributors
trvrb
chacalle
barneypotter24
jameshadfield
rneher
Latest commits
Pages

DENGUE Pipeline Notes

Upload

ViPR sequences

  1. Download sequences
    • Select genome length >= 5000
    • Download as Genome Fasta
    • Set Custom Format Fields to 0: GenBank Accession, 1: Strain Name, 2: Segment, 3: Date, 4: Host, 5: Country, 6: Subtype, 7: Virus Type
  2. Move downloaded sequences to fauna/GenomicFastaResults.fasta
  3. Upload to vdb database
    • python2 vdb/dengue_upload.py -db vdb -v dengue --source genbank --locus genome --fname GenomicFastaResults.fasta

Update

  • Update citation fields
    • python2 vdb/dengue_update.py -db vdb -v dengue --update_citations
    • updates authors, title, url, journal and puburl fields from genbank files
    • If you get ERROR: Couldn't connect with entrez, please run again just run command again

Download sequence documents from VDB

  • python2 vdb/dengue_download.py # all serotypes together
  • python2 vdb/dengue_download.py --select serotype:dengue_virus_1 # just serotype 1
  • python2 vdb/dengue_download.py --select serotype:dengue_virus_2 # just serotype 2
  • python2 vdb/dengue_download.py --select serotype:dengue_virus_3 # just serotype 3
  • python2 vdb/dengue_download.py --select serotype:dengue_virus_4 # just serotype 4

Download titer documents from TDB

  • python2 tdb/download.py -db tdb -v dengue --fstem dengue