Dengue antigenic variation and evolutionary dynamics


Nextstrain is an open-source project to harness the scientific and public health potential of pathogen genome data. We provide a continually-updated view of publicly available data with powerful analytics and visualizations showing pathogen evolution and epidemic spread. Our goal is to aid epidemiological understanding and improve outbreak response.

Resulting data and inferences are available live at the website Documentation is available at


Definition: One held to foretell events by omens.

Augur is the bioinformatic processing pipeline to track evolution from sequence and serological data. Documentation for augur is available at


To install augur, clone the git repository and its submodules

git clone
cd augur
git submodule update --init

Augur has a number of python dependencies that are listed in requirements.txt and are best installed via a package manager like conda or pip.

pip install -r requirements.txt

Augur is written in Python 2.7 and requires Python 2.7 to run. Your version of Python can be confirmed by running python --version.

In addition, augur needs working installations of mafft and one of the following tree builders

  • DEFAULT: RAxML. You’ll probably need to create a symlink raxml -> raxmlHPC because augur expects an excutable named raxml.
  • OPTIONAL: FastTree

Virus builds

Each virus build consists of a and file. Currently supported builds are listed in the builds directory.

Copyright 2014-2018 Trevor Bedford and Richard Neher.

Source code to Nextstrain is made available under the terms of the GNU Affero General Public License (AGPL). Nextstrain is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU Affero General Public License for more details.